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. 2014 Jan 6;9(1):e84374. doi: 10.1371/journal.pone.0084374

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© 2014 Ezkurdia et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Magnifications (400×) of the ventral horn area of the spinal cord (middle gray color image at 4×), containing cholinergic neurons, showing strong VAChT immunofluorescent signal and null immunoreactivity for Sema3A. (B) Magnifications (400×) of the spinal cord intermediolateral nucleus containing cholinergic preganglionic sympathetic neurons. These neurons show positive immunofluorescent signal for both VAChT and Sema3A. HE, hematoxilin-eosin staining.