Figure 8. Evaluation of the compounds' capacity to delay PrP^Sen conversion into PrP^Res by RT-QuIC assay seeded with 263 K scrapie.

RT-QuIC reactions were seeded with 10fg of 263 K infected hamster brain or the equivalent amount of normal brain homogenates (NBH). The substrate for the reaction was recombinant hamster PrP^90–231. NaCl was used at 300 mM final concentration. Compounds Y13 and Y17 were assayed at 25 and 50 μM. The assay was followed by ThT fluorescence (excitation 450 nm; emission 480 nm) emission over time (average of 4 replicate wells).