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(A) Rspo1 and Rspo2 mRNA expression was measured by qPCR in ISEMFs that were supportive or non-supportive of crypt growth without exogenous Rspo1 (n = 3). (B) Enteroid forming efficiency was measured in co-cultures with supportive or non-supportive ISEMFs, with and without exogenous Rspo2 in the culture media. Asterisk indicates p<0.05 when compared to Supportive ISEMF in (A) and between the indicated groups in (B).
