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. 2014 Jan 6;9(1):e84818. doi: 10.1371/journal.pone.0084818

Figure 1. Effect of MKK-deficiency in osteoclastogenesis in vitro.

Figure 1

(A) WT, MKK3−/− and MKK6−/− bone marrow cells were cultured to induce osteoclastogenesis and analyzed by TRAP staining (n = 3/group). The number of osteoclasts were counted in 5 pre-defined locations per well and the data are represented as mean ± SEM. Numbers of osteoclasts were reduced in SB-treated WT and MKK3−/− cells compared with WT. Osteoclastogenesis in MKK6−/− group were comparable to WT cells. *p<0.05 (B) Phosphorylation of p38 in MKK-deficient osteoclasts. Western blot analysis showed that P-p38 levels were significantly lower in MKK3−/− but not in MKK6−/− compared with WT group. *p<0.05.