Figure 2. Bone resorption by MKK3- and MKK6-deficient osteoclasts.

WT, MKK3−/− and MKK6−/− bone marrow cells were differentiated into osteoclasts on a calcium matrix and subjected to Von Kossa staining to determine resorption activity. The resorption pit area was calculated in eight areas/well and data are shown as percent resorbed area/total area. Resorption activity was significantly reduced in MKK3−/− but not MKK6−/− osteoclasts compared with WT. n = 3/group, *p<0.05.