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. Author manuscript; available in PMC: 2014 Oct 22.
Published in final edited form as: Curr Opin Immunol. 2013 Oct 22;25(5):10.1016/j.coi.2013.09.010. doi: 10.1016/j.coi.2013.09.010

Table 1.

Characteristics of three of the most commonly used current next-generation sequencing techniques

Roche 454 GS FLX Titanium* Illumina MiSeq** Illumina HiSeq 2500‡
Read Length Up to 600 bp Up to 500 bp (250 × 2) Up to 200 bp (100 × 2)
Output 450 Mb 7.5–8.5 Gb 540–600 Gb
Reads Run 700,000 15 Million 3 Billion
Quality Consensus accuracy of 99.995% > 75% bases above Q30 > 80% bases above Q30
*

“GS FLX+ System.” 454 Life Sciences, a Roche Company. Web. Accessed 05 Aug 2013.

**

“MiSeq Benchtop Sequencer Specifications.” Illumina: sequencing and array-based solutions for genetic research. Web. Accessed 05 Aug 2013.

‡

“HiSeq 2500/1500 Specifications.” Illumina: sequencing and array-based solutions for genetic research. Web. Accessed 05 Aug 2013.