Fig. 5.
γE531 restrains the activity of wild-type channels. A: sequence alignments of the swapped regions within γ-βTM2 and the wild-type γ-subunit. Identical residues are indicated with a grey background. TM2 residues are within the box. The degenerin (DEG) site, putative amiloride binding site, and selectivity filter are noted. B: amiloride-sensitive Iss/Ipeak values of channels with γ-βTM2, γ-βTM2 mutants, a wild-type γ-subunit, or γ-subunit mutants are shown (n = 9 to 24 for each group from a minimal of 2 batches of oocytes; nd, not determined). *P < 0.05, channels with an Iss/Ipeak value significantly different than the αβγ-βTM2 channel. #P < 0.05, channels with an Iss/Ipeak value significantly different than the αβγ-channel. Statistical significance was determined by one-way ANOVA followed by a Bonferroni test.