Fig. 1.

Bone marrow-derived angiogenic cell (BMAC) function can be improved significantly by thrombospondin-1 (TSP-1) deletion. BMACs were isolated from TSP-1^−/− mice and their wild-type (WT) controls. BMAC function was evaluated by Matrigel tube formation, migration, and adhesion. A: TSP-1^−/− BMAC formed a significantly better tube network on Matrigel compared with WT controls. B: bar graph of accumulated tube length form by WT and TSP-1^−/− BMACs; n = 6/group. C: bar graph of WT and TSP-1^−/− BMAC adhesion; n = 6/group. D: bar graph of WT and TSP-1^−/− BMAC adhesion; n = 6/group. E: Western blot analysis of TSP-1 protein expression in WT and TSP-1^−/− mice; n = 6/group. Representative bands are shown at right. *P < 0.05 vs. WT.