Fig. 2.

A: time course of ETEC effect on thiamin uptake by confluent Caco-2 monolayers. Caco-2 cells were infected with ETEC for 1, 2, and 3 h, followed by postinfection incubation of 6 h. *P < 0.01, compared with control. B: effects of postinfection time on thiamin uptake by Caco-2 cells. Caco-2 monolayers were infected with ETEC for 3 h and postincubated for 1, 3, 6, and 10 h. Thiamin uptake was measured as described in Methods. Values are means ± SE of 3 separate uptake determinations. *P < 0.01, compared with control.