Table 1.
Antibodies, concentration, and source
| Antibody | Dilution | Source | Location |
|---|---|---|---|
| Rabbit anti-PDGFR-β | 1:250 | Dr. W. Stallcup | Sanford-Burnham Medical Research Institute, CA |
| Rat anti-mouse CD146 | 1:250 | BioLegend (cat. no. 134702) | San Diego, CA |
| Rat anti-CD31 (PECAM-1) | 1:100 | BD Biosciences (cat. no. 558736) | San Jose, CA |
| Rabbit anti-type I collagen | 1:1,000 | AbD Serotec (cat. no. 2150–1410) | Raleigh, NC, |
| Mouse anti-MHC (MF 20) | 1:2,000 | Developmental Studies Hybridoma Bank, University of Iowa | Iowa City, IA |
| Rabbit anti-NG2 Chondroitin sulfate proteoglycan | 1:100 | Chemicon-Millipore (cat. no. AB5320) | Temecula, CA |
| Mouse anti-Pax7 | 1:100 | Developmental Studies Hybridoma Bank, University of Iowa | Iowa City, IA |
| Mouse anti-FSP1 | 1:100 | Novus Biologicals (cat. no. H00006275-M01) | Littleton, CO |
| Mouse anti-fast myosin skeletal heavy chain | 1:100 | Abcam (cat. no. ab51263) | Cambridge, MA |
PDGFR-β, platelet-derived growth factor receptor-β; PECAM-1, platelet endothelial cell adhesion molecule-1; MHC, myosin heavy chain; FSP1, fibroblast-specific protein 1; NG2, neuron-glial antigen 2.