Fig. 7.
Time course of the effect of 10 μM amiloride on the recovery of INa following exposure to HS (660 mosmol/kgH2O) under near “thin-film” conditions in CF-hBE cells. The inserts were exposed to 30 μl HS (660 mosmol/kgH2O) in either the absence (○, n = 6) or presence (●, n = 6) of amiloride. At the various indicated times the inserts were washed with isosmotic buffer and mounted on Ussing chambers to measure INa under isosmotic conditions. SEs are shown where values extend beyond symbols (see text for further details).