Fig. 2.

Phenethyl isothiocyanate (PEITC) and pyrrolidine dithiocarbamate (PDTC) suppress NF-κB activation in cyp1b1^−/− retinal vascular cells. A: cyp1b1^−/− EC were incubated with PEITC (500 nM and 1 μM) or PDTC (5, 10, and 50 nM), and lysates were analyzed by Western blot analysis for phosphorylated p65 (p-p65), total p65, and β-actin. B: quantitative assessment of data in A (n = 3). **P < 0.01, ***P < 0.001. C: cyp1b1^−/− PC were incubated with PEITC (500 nM and 1 μM) or PDTC (5, 10, and 50 nM), and lysates were analyzed by Western blot analysis for phosphorylated p65, total p65, and β-actin. D: quantitative assessment of data in C (n = 3). *P < 0.05. E and F: NF-κB activity was determined in cyp1b1^+/+ and cyp1b1^−/− EC and PC using an NF-κB luciferase reporter assay in the presence or absence of PEITC or PDTC (n = 3). **P < 0.01, ***P < 0.001, ****P < 0.0001. G: indirect immunofluorescent staining using p65 was performed to demonstrate p65 NF-κB localization; 4′,6-diaminido-2-phenylindole was used to stain cell nuclei. Scale bar, 50 μm.