Fig. 3.
HMGB1 is essential for CMEtOH-induced migration of HSC and LEC. In Boyden chamber assay, CMEtOH-induced cell migration was blocked by HMGB1-neutralizing antibody (1 μg/ml) in HSC (A) and LEC (B). HepG2 cells were transfected with HMGB1-siRNA. HMGB1 protein expression was markedly decreased by HMGB1-siRNA transfection, and nuclear (N) levels were reduced in response to ethanol based on Western blot assay. GAPDH blot is shown from nuclear and cytosolic (C) preparations (C). CMEtOH from HMGB1-siRNA-transfected cells did not induce migration of HSC (D) or LEC (E), whereas the number of migrated cells was significantly increased by CMEtOH from control-siRNA-transfected cells. Results depicted are compiled from at least 3 experiments. Data represents means ± SE. *P < 0.05.