Fig. 4.

HMGB1 induces Src and Erk phosphorylation in HSC. Serum-starved HSC and LEC were treated with recombinant HMGB1 (100 ng/ml) for different durations (0–90 min). Total cell lysates were used for Western blot to detect phosphorylated Src and Erk. Src and Erk phosphorylation were significantly increased in HSC in response to 15-min treatment with HMGB1 (A) but not in LEC (B). *P < 0.05. Blots are representative of at least 3 replicates. Graph depicts data compiled from experiments and replicates in total. IB, immunoblotting.