Fig. 5.

Src contributes to HMGB1-induced HSC migration. Boyden chamber assay was performed on HSC and LEC exposed to recombinant HMGB1 (100 ng/ml). PP2 (10 μM) pretreatment for 30 min blocked HMGB1-induced migration of HSC (A) but did not affect HMGB1 migration of LEC (B). HSC and LEC were transfected with Src mutant retroviral construct (Src-Y419F) or Src-wild type (Src-WT) retrovirus. In Boyden chamber assay, cell migration was increased in response to recombinant HMGB1 in Src-WT HSCs, but not Src-Y419F mutant HSCs (C). Src-Y419F mutant construct transfection did not affect HMGB1 migration of LEC (D). Results were obtained from at least 3 independent experiments. Data in bar graphs represent means ± SE and are expressed as number of cells in the filter. *P < 0.05.