Table 3.

Levels of specialized proresolving and proinflammation mediators in liver tissue of mice fed PN with SOLE or FOLE

	Pathway Marker Transitions		LM, pg/500 mg liver
	Quartile 1	Quartile 3	HCD	SOLE	FOLE
DHA bioactive metabolome
RvD1	375	215	*	*	*
RvD2	375	141	*	*	*
RvD3	375	147	*	*	*
RvD5	359	199	*	*	*
RvD6	359	159	*	*	*
PD1	359	153	*	*	*
10S,17S-diHDHA	359	153	222 ± 93	173 ± 20	493 ± 217
10-epi-Δ15-trans-PD1	359	153	38.4 ± 17.5	20.5 ± 0.03	72.7 ± 46.8
Δ15-trans-PD1	359	153	25.2 ± 11.0	24.4 ± 2.7	68.6 ± 27.3
22-OH-PD1	375	153	*	*	*
22-COOH-PD1	389	153	*	*	*
MaR1	359	250	*	*	*
7S,14S-diHDHA	359	250	50.3 ± 29.7	49.9 ± 7.1	233 ± 120
7-epi-Δ12-trans-MaR1	359	250	74.3 ± 35.5	77.8 ± 6.9	136 ± 40
Δ12-trans-MaR1	359	250	28.7 ± 15.1	46.6 ± 0.3	99.5 ± 24.8
4S,14S-diHDHA	359	101	80.8 ± 39.1	41.6 ± 16.2	250 ± 101
DHA pathway markers
17-HDHA	343	245	383 ± 64	243 ± 58	1,006 ± 141†§§
14-HDHA	343	205	2,891 ± 311	2,375 ± 527	6,363 ± 1,300§
7-HDHA	343	141	47.9 ± 15.2	35.0 ± 3.0	145 ± 32†§
4-HDHA	343	101	124 ± 32	68.0 ± 12.0	309 ± 89
EPA bioactive metabolome
RvE1	349	195	*	*	*
RvE2	333	253	*	*	*
RvE3	333	201	*	*	*
LXA5	349	215	*	*	*
LXB5	349	221	*	*	*
5S,15S-diHEPE	333	253	61.0 ± 50.7	30.4 ± 15.2	471 ± 399
EPA pathway markers
18-HEPE	317	259	35.4 ± 21.6	323 ± 113	4,678 ± 388†††§§§
15-HEPE	317	219	93.8 ± 54.9	480 ± 157	2,543 ± 152†††§§§
12-HEPE	317	179	311 ± 151	3,042 ± 691	2,2809 ± 5,514††§
5-HEPE	317	115	14.8 ± 0.8	59.9 ± 15.5	337 ± 43†††§§§
AA bioactive metabolome
LXA4	335	115	*	*	*
LXB4	351	221	*	*	*
5S,15S-diHETE	335	235	96.8 ± 37.3	203 ± 28	162 ± 65
LTB4	335	195	*	*	*
5S,12S-diHETE	335	195	16.3 ± 5.1	19.1 ± 4.5	69.9 ± 34.6
12epi-Δ6-trans-LTB4	335	195	11.1 ± 5.0	8.0 ± 1.0	2.6 ± 1.3
Δ6-trans-LTB4	335	195	13.3 ± 5.2	10.9 ± 0.4	3.3 ± 1.6
20-OH-LTB4	351	195	*	*	*
20-COOH-LTB4	365	195	*	*	*
PGD2	351	189	770 ± 489	792 ± 168	198 ± 72
PGE2	351	189	525 ± 393	197 ± 16	123 ± 56
PGF2α	353	193	925 ± 413	1,731 ± 668	283 ± 105
TXB2	369	169	60.8 ± 45.8	24.6 ± 5.6	17.2 ± 6.3
AA pathway markers
15-HETE	319	219	681 ± 135	1,383 ± 374	792 ± 85
12-HETE	319	179	3,325 ± 374	5,051 ± 769	3,302 ± 246
5-HETE	319	115	138 ± 29	113 ± 10	52.4 ± 3.7†
Sum of proresolving mediators			677 ± 250	667 ± 19	1,984 ± 1,040
Sum of proinflammatory mediators			2,261 ± 1,130	2,758 ± 831	680 ± 253
Proresolution index			0.3	0.2	2.9
(sum of proresolving mediators/sum of inflammatory mediators)

LM values are means ± SE (n = 3). Quartile 1, M-H (parent ion); quartile 3, diagnostic ions in the MS-MS (daughter ion).

^*Below limits of detection (detection limit was ∼2 pg in tissue matrix). Ion suppression effect was 85–92%.

^†P < 0.05,

^††P < 0.01,

^†††P < 0.001 vs. HCD.

^§P < 0.05,

^§§P < 0.01,

^§§§P < 0.001 vs. SOLE.