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. 2013 Oct 10;305(12):G919–G932. doi: 10.1152/ajpgi.00226.2013

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Fig. 4. — Hepatic inflammation in rats after ethanol feeding with different dietary fats for 8 wk. A: macrophages in the liver detected by immunofluorescence staining. Scale bar: 20 μm. Red: CD68⁺ or CD163⁺ macrophages; blue: 4′,6-diamidino-2-phenylindole (DAPI) counterstaining of nuclei. B: quantitative analysis of CD68⁺ and CD163⁺ macrophages in the liver by Image Pro Premier (n = 25–35). C: hepatic cytokine/chemokine expressions of cxcl1/CINC-1 and ccl2/MCP-1 measured by quantitative PCR (qPCR). The relative gene expression was normalized to 18s rRNA expression and calculated by the 2^−ΔΔCt method setting the value of PF/CO as 1. Results are means ± SD (n = 6). Significant differences (P < 0.05, ANOVA) are indicated by different letters.