Fig. 2.

Western blots of rabbit renal PT preparations. Tubules were exposed to our control (Ctrl) 5% CO₂/22 mM HCO₃⁻ solution for 5 min, and aliquots of extracted protein were resolved by SDS-PAGE, transferred to PVDF membranes, probed with the indicated phosphotyrosine (pY) antibodies, stripped, and then reprobed with an antibody to ErbB1 or ErbB2. A: blots were probed first with anti-pan-pY (left) and reprobed with anti-ErbB1 (right). B: blots were probed first with anti-ErbB1/pY845. C: blots were probed first with anti-ErbB1/pY992. D: blots were probed first with anti-ErbB1/pY1068. E: blots were probed first with anti-ErbB1/pY1173. F: blots were probed first with anti-ErbB2/pY1221/pY1222.