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Per1 knockdown results in decreased 3β-HSD expression in adrenal cells in vitro. NCI-H295R cells were treated with nontarget or Per1 small interfering (si)RNA for 48 h. Quantitative PCR was used to evaluate changes in Per1 (A), 3β-HSD (B), or CYB11 (C) expression in Per1 siRNA versus the nontarget siRNA control. Values are presented as means ± SE; n = 3. *P < 0.05; **P < 0.01.
