Fig. 4.

Pharmacological blockade of Per1 nuclear entry results in decreased 3β-HSD in vivo. Weight-matched male WT 129/sv mice were given either vehicle (20% hydroxypropyl-β-cyclodextrin) or 30 mg/kg PF-670462 subcutaneously every 12 h for 2.5 days starting at noon and euthanized at midnight 12 h after the last injection, as previously published and described (27). Adrenal glands were harvested, and 3β-HSD (A) and CYPB11 (B) mRNA expression was measured by quantitative PCR. Values are presented as means ± SE; n = 4. *P < 0.05 compared with WT mice.