Fig. 7.

Podocyte injury induces macrophage infiltration in vitro. A Transwell migration assay was performed. Podocytes were plated and cultured in six-well plates in culture medium with either normal glucose (NG) or high-glucose (HG) media as indicated. Macrophages were cultured in the upper chamber. Mouse IgG was used as the negative control, whereas mouse monocyte chemoattractant protein (MCP)-1 was used as positive control. Anti-mouse MCP-1 (α-MCP-1) antibody was added to neutralize MCP-1 as an additional control. Mannitol was used as the osmotic control. Sixteen hours after coculture with podocytes, migrated macrophages were counted, and percent changes of macrophage migration were determined. Results are means ± SE. *P < 0.05 compared with NG medium; #P < 0.01 compared with HG medium.