Figure 3.

Determination of the second order fibril extension rate constant, k⁺. (a) Typical fibril growth reaction progress curves of reactions seeded by fibril samples taken from the fragmentation reaction shown in Fig. 1, monitored by ThT fluorescence. All curves are normalized to their upper baselines (unnormalized traces are shown in Fig S2). The times each sample was fragmented, before addition to excess monomer to stimulate fibril growth, are shown to the right. (b) Initial slope of the normalized reaction progress curves versus fibril seed particle concentration. The error bars indicate one standard error of four reaction replicates. The red line denotes a linear function fit to the data using a total least squares method. To see this figure in color, go online.