Figure 1.

(a) Baseline-corrected titration calorimetry of 5 μL injections of 2.3 mM calcium binding to a solution of 1.463 mM EDTA chelator in pH 7.4, 25 mM TRIS, 150 mM KCl buffer at 25°C with 1.106 mM of added calcium. (b) Fitted isotherm of panel a, using the fitted parameters from the ODE implementation of a single-site binding model with a 1.106-mM residual ligand and purity term. Residual sum-of-squares (RSS; cal/mol) are shown below the fitted isotherm.