Table 3.
Fitted parameters and F-test-derived confidence intervals for simultaneously simulated isotherms of calcium binding to EF-hand proteins with and without 5,5′-dimethylBAPTA (P = 0.05)
| Macromolecule (trial No.) | 5,5′-dimethylBAPTA (fixed parameters) |
Purity (%) | ΔH (cal/mol) | KD (μM) | Residual ligand (μM) | ||
|---|---|---|---|---|---|---|---|
| Purity (%) | ΔH (cal/mol) | KD (nM) | |||||
| 15N HuPC2EF (1) | 73 | 2180 | 125 | 100 (fixed) | −11,237 (−11,568–10,913)a | 461 (437–486) | 2.39 (2.01–2.80) |
| 15N SuPC2EF-x-z1 (1) | 73 | 2275 | 125 | 100 (fixed) | −20,071 (−19,702–20,442) | 1.95 (1.78–2.15) | 10.8 (10.5–11.1) |
a
Parentheses indicate 95% confidence intervals for floating parameters, computed from sum-of-squares best-fit values; they do not reflect propagated experimental errors.