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. 2013 Dec 17;105(12):2733–2742. doi: 10.1016/j.bpj.2013.11.002

Figure 2.

Figure 2

Detection of domain formation in vesicles composed of DSPC/DMoPC/sterol by FRET. MLVs (500 μM total lipid) composed of DMoPC with 45 mol % cholesterol (Inline graphic), DMoPC with 45 mol % 1:1 coprostanol/epicholesterol (Inline graphic), 1:1 DSPC/DMoPC with 45 mol % cholesterol (▪), 1:1 DSPC/DMoPC with 45 mol % 1:1 cholesterol/epicholesterol (Inline graphic), or 1:1 DSPC/DMoPC with 45 mol % 1:1 coprostanol/epicholesterol (♦) were prepared in PBS pH 5.1. F samples contained both FRET donor (0.05 mol % pyrene-DPPE) and FRET acceptor (2 mol % Rho-DOPE). Fo samples only contained FRET donor (0.05 mol % pyrene-DPPE). The ratio of donor fluorescence in the presence of acceptor to that in its absence (F/Fo) is graphed. Average (mean) values and SD values from triplicates are shown. Abbreviations: chol = cholesterol; epichol = epicholesterol; and cop = coprostanol.