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. 2013 Dec 17;105(12):2655–2665. doi: 10.1016/j.bpj.2013.11.012

Figure 2.

Figure 2

Dynamics of Notch1-eGFP cluster depletion. (A) Representative TIRFM image of Notch1-eGFP taken from a time-lapse series showing the depletion dynamics from a single cell. Two clusters are highlighted: position 1 shows attenuation of eGFP signal, whereas position 2 shows only small fluctuations in signal intensity. (B) Signal quantification using line scans taken through both clusters. Dashed line indicates intensity profile at t = 52 min. (C) Representative fluorescence image showing sudden loss of an entire Notch1-eGFP cluster and (D) line scans taken through two clusters to highlight this event. Scale bar = 5 μm. To see this figure in color, go online.