Fig. 5.
E2 rapidly stimulated ENaC NPo in alveolar cells through G protein-coupled estrogen receptor (Gper). To determine if and how E2 exhibited rapid effects on ENaC activity, we measured ENaC single channel activity immediately preceding (Pre) and after application of E2 or the estrogen receptor agonists, 2,3-bis(4-hydroxyphenyl)propionitrile (DPN) and rel-1-[4-(6-bromo-1,3-benzodioxol-5-yl)-3aR,4S,5,9bS-tetrahydro-3H-cyclopenta[c]quinolin-8-yl]-ethanone (G-1) (Post). Black symbols represent data from an individual patch before and after treatment, connected by a dotted line. Gray circles represent the mean ± SE of these points pre- and posttreatment, connected by a solid line. A: a representative recording of channel activity before and after E2 treatment is shown. B: E2 significantly increased ENaC NPo within minutes after application to the bath. C: representative single channel recordings are shown from L2 cells before and after treatment with 10 nM DPN (ERβ agonist) or 10 nM G-1 (Gper agonist). D: NPo did not change with application of DPN (n = 4) but was significantly increased with G-1 (n = 6) treatment. *P < 0.05 vs. Pre as determined by one-sided paired t-test.