Fig. 2.

Superoxide produced in lungs with stop of flow [as detected by 2-hydroxyethidium (2-OHE)]. A: elution traces of 2-OHE, produced by xanthine/xanthine oxidase by HPLC. B: sample elution traces of 2-OHE in supernatant from lungs subjected to 1 h stop of flow (HE signal not shown). C: quantification of oxidized dihydroethidium. Dashed line shows 2-OHE in naïve (untreated) lungs. Data are ratio of peak density of 2-OHE over HE. Data are means ± SE for n > 3 lungs. * P < 0.05 compared with WT.