Fig. 2.
Effect of ASHMI on mouse ex vivo tracheal ring contractility. A: tracheal contractility to 10−4 M ACh in the presence of various concentrations of ASHMI normalized to maximal constriction to 60 mM KCl. Values are means ± SD of 4–7 individual responses for each ASHMI concentration. *P < 0.05 and ***P < 0.001 compared with no ASHMI. B: representative real-time tracings of tracheal rings from OVA-sensitized mice stimulated with 10−4 M ACh after pretreatment with 200 μg/ml ASHMI (center panel, bottom trace) or physiological salt solution (PSS; vehicle; center panel, top trace). KCl (60 mM) was used to determine the maximum contractility of rings before ACh exposure and to confirm the viability of rings after experiments. In some experiments, 10−4 M ACh was used to determine reversibility of ASHMI effects (bottom panel).