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Effect ASHMI on PGE2 levels. PGE2 levels in medium conditioned by intact- or epithelium-denuded tracheal rings pretreated with ASHMI (200 μg/ml) or medium alone (Med), and then subsequently stimulated with 10−4 M ACh or medium alone for 20 min, were measured by ELISA. Values are means ± SD of 4 separate experiments. *P < 0.05. **P < 0.01. ***P < 0.001.
