Table 1.
A summary of crosslinkers used in the analysis of receptor protein interactions.
| Chemical name1 | Membrane permeable | Reversible | Reactive toward | Receptor interactome applications | Spacer arm |
|---|---|---|---|---|---|
| DSS | Y | N | Amines | Gardoni etal. (2002), Wang etal. (2002),Chu etal. (2004) | 11.4 |
| DSP | Y | Y; by DTT | Amines | Luttrell etal. (1999), Lau and Hall (2001), Quian etal. (2001), Shenoy etal. (2006) | 12.0 |
| BS3 | N | N | Amines | Aldecoa etal. (2000), Nordman and Kabbani (2012) | 11.4 |
| *MBP | Y | N | Sulfydryls | – | <5 |
| *ANB-NOS | Y | N | Amines | – | 7.7 |
| *Sulfo-SAED | N | Y; by DTT | Amines | Yun-Hong etal. (2011) | 23.6 |
*Denotes photo-reactive linkers.
1
IUPAC Names: DSS, disccinimidyl suberate; DSP, dithiobis(succinimidyl propionate; BS3, bis(sulfosuccinimidyl) suberate; MBP, 4-maleimidobenzophenone; ANB-NOS, N-5-azido-2-nitrobenzoyloxysuccinimide; SAED, sulfosuccinididyl 2-(7-azido-4-methylcoumarin-3 -acetoadmio)-ethyl-1,3 ′-dithiopropionate.