Table 2.
RT-PCR results
| 70d RUN | 70d SED | 77d RUN | 77d 7d-WL | 77d SED | |
|---|---|---|---|---|---|
| Omental | |||||
| TNF-α | 1.00 ± 0.28 | 1.26 ± 0.51 | 0.75 ± 0.16 | 0.68 ± 0.06 | 0.95 ± 0.15 |
| IL-6 | 1.00 ± 0.41a | 2.49 ± 0.40b | 0.72 ± 0.15a | 0.64 ± 0.14a | 2.22 ± 0.40b |
| MCP-1 | 1.00 ± 0.19 | 1.08 ± 0.17 | 1.08 ± 0.16 | 1.48 ± 0.38 | 1.79 ± 0.43 |
| HIF-1α | 1.00 ± 0.14a | 1.53 ± 0.21a | 1.08 ± 0.12a | 0.93 ± 0.13a | 1.43 ± 0.27b |
| VEGF | 1.00 ± 0.25 | 0.88 ± 0.21 | 1.05 ± 0.15 | 0.69 ± 0.16 | 1.23 ± 0.26 |
| Leptin | 1.00 ± 0.22a,b | 1.53 ± 0.20a | 0.69 ± 0.19b | 0.75 ± 0.19b | 1.51 ± 0.25a |
| Adiponectin | 1.00 ± 0.15 | 0.61 ± 0.11 | 0.79 ± 0.16 | 0.66 ± 0.14 | 0.78 ± 0.27 |
| Cyclin A1 | 1.00 ± 0.15 | 0.85 ± 0.37 | 0.93 ± 0.34 | 0.63 ± 0.15 | 0.92 ± 0.30 |
| CDK2 | 1.00 ± 0.10 | 0.81 ± 0.10 | 1.06 ± 0.11 | 0.96 ± 0.09 | 1.15 ± 0.26 |
| PPAR-γ | 1.00 ± 0.22a | 0.53 ± 0.07b,c | 0.68 ± 0.12a,c | 0.43 ± 0.09b,c | 1.07 ± 0.18a |
| C/EBPα | 1.00 ± 0.22 | 0.82 ± 0.16 | 0.66 ± 0.11 | 0.69 ± 0.21 | 1.41 ± 0.39 |
| Epididymal | |||||
| TNF-α | 1.00 ± 0.11 | 1.83 ± 0.70 | 1.15 ± 0.22 | 1.88 ± 0.38 | 1.21 ± 0.26 |
| IL-6 | 1.00 ± 0.13a | 3.65 ± 0.68b | 2.03 ± 0.34a | 1.40 ± 0.33a | 2.58 ± 0.64a,b |
| MCP-1 | 1.00 ± 0.13a | 2.03 ± 0.41b | 1.21 ± 0.14a | 1.64 ± 0.21a,b | 1.45 ± 0.23a,b |
| HIF-1α | 1.00 ± 0.05a | 2.16 ± 0.19b | 0.98 ± 0.17a | 1.96 ± 0.27b | 1.72 ± 0.32b |
| VEGF | 1.00 ± 0.07 | 0.72 ± 0.10 | 1.43 ± 0.21 | 0.94 ± 0.17 | 0.89 ± 0.24 |
| Leptin | 1.00 ± 0.13 | 1.70 ± 0.37 | 0.76 ± 0.15 | 1.15 ± 0.11 | 1.38 ± 0.26 |
| Adiponectin | 1.00 ± 0.06 | 1.20 ± 0.17 | 0.85 ± 0.15 | 1.11 ± 0.09 | 1.19 ± 0.33 |
| Cyclin A1 | 1.00 ± 0.10a | 2.49 ± 0.26b | 1.10 ± 0.07a | 1.72 ± 0.16c | 2.24 ± 0.38b,c |
| CDK2 | 1.00 ± 0.08 | 1.15 ± 0.15 | 0.97 ± 0.16 | 1.14 ± 0.14 | 1.15 ± 0.15 |
| PPAR-γ | 1.00 ± 0.08 | 0.90 ± 0.26 | 1.04 ± 0.07 | 0.79 ± 0.08 | 1.09 ± 0.37 |
| C/EBPα | 1.00 ± 0.08 | 0.69 ± 0.22 | 1.08 ± 0.08 | 0.79 ± 0.13 | 0.86 ± 0.26 |
| Perirenal | |||||
| TNF-α | 1.00 ± 0.10 | 1.29 ± 0.14 | 0.99 ± 0.05 | 1.26 ± 0.17 | 1.19 ± 0.16 |
| IL-6 | 1.00 ± 0.18a | 2.84 ± 0.19b | 2.40 ± 0.18b | 1.35 ± 0.16a | 2.74 ± 0.52b |
| MCP-1 | 1.00 ± 0.18 | 1.62 ± 0.25 | 1.17 ± 0.24 | 1.42 ± 0.15 | 1.51 ± 0.31 |
| HIF-1α | 1.00 ± 0.06 | 1.07 ± 0.17 | 1.17 ± 0.12 | 1.22 ± 0.07a | 1.08 ± 0.19 |
| VEGF | 1.00 ± 0.08a,b | 0.88 ± 0.21b | 1.46 ± 0.20a | 1.15 ± 0.17a,b | 0.74 ± 0.15b |
| Leptin | 1.00 ± 0.16a,c | 1.69 ± 0.23b | 0.80 ± 0.19a | 0.85 ± 0.13a | 1.38 ± 0.21b,c |
| Adiponectin | 1.00 ± 0.05 | 0.96 ± 0.12 | 0.79 ± 0.08 | 0.86 ± 0.11 | 0.82 ± 0.12 |
| Cyclin A1 | 1.00 ± 0.08a | 2.16 ± 0.31b | 1.14 ± 0.22a | 1.78 ± 0.09b | 2.10 ± 0.30b |
| CDK2 | 1.00 ± 0.08 | 1.14 ± 0.38 | 0.91 ± 0.14 | 1.00 ± 0.10 | 0.88 ± 0.13 |
| PPAR-γ | 1.00 ± 0.10 | 1.08 ± 0.18 | 1.17 ± 0.08 | 0.93 ± 0.08 | 0.89 ± 0.20 |
| C/EBPα | 1.00 ± 0.08 | 0.62 ± 0.15 | 0.91 ± 0.12 | 0.92 ± 0.17 | 0.78 ± 0.19 |
| Subcutaneous | |||||
| TNF-α | 1.00 ± 0.28 | 1.06 ± 0.17 | 0.97 ± 0.24 | 0.87 ± 0.09 | 0.90 ± 0.11 |
| IL-6 | 1.00 ± 0.29 | 0.63 ± 0.30 | 1.27 ± 0.33 | 0.61 ± 0.13 | 1.16 ± 0.29 |
| MCP-1 | 1.00 ± 0.17 | 0.86 ± 0.33 | 1.24 ± 0.22 | 1.49 ± 0.25 | 1.35 ± 0.22 |
| HIF-1α | 1.00 ± 0.07 | 0.85 ± 0.12 | 0.94 ± 0.08 | 1.02 ± 0.20 | 0.85 ± 0.16 |
| VEGF | 1.00 ± 0.08 | 0.74 ± 0.14 | 1.21 ± 0.14 | 1.22 ± 0.34 | 0.84 ± 0.30 |
| Leptin | 1.00 ± 0.40 | 1.09 ± 0.47 | 1.07 ± 0.36 | 1.13 ± 0.30 | 1.03 ± 0.21 |
| Adiponectin | 1.00 ± 0.25 | 0.90 ± 0.17 | 0.97 ± 0.17 | 1.29 ± 0.35 | 0.89 ± 0.34 |
| Cyclin A1 | 1.00 ± 0.21a | 1.95 ± 0.35b | 0.98 ± 0.26a | 1.86 ± 0.19b | 2.08 ± 0.31b |
| CDK2 | 1.00 ± 0.14 | 0.95 ± 0.20 | 0.98 ± 0.13 | 1.06 ± 0.21 | 1.09 ± 0.26 |
| PPAR-γ | 1.00 ± 0.22 | 0.74 ± 0.13 | 1.02 ± 0.19 | 1.21 ± 0.33 | 0.78 ± 0.18 |
| C/EBPα | 1.00 ± 0.19 | 0.97 ± 0.11 | 0.76 ± 0.16 | 1.06 ± 0.34 | 0.74 ± 0.26 |
Values are means ± SE. mRNA expression in at-sexually mature rats. mRNA expression values are presented as 2ΔΔCT whereby ΔCT = B2M CT − gene of interest CT and were normalized to 1.0 for 70d RUN. The genes in italics highlight genes that showed a wheel lock (WL) effect (i.e., 77d 7d-WL was different than 77d RUN). Between-group differences are noted as by different letters, P < 0.05.