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. 2013 Oct 15;45(23):1168–1185. doi: 10.1152/physiolgenomics.00022.2013

Fig. 3.

Fig. 3.

Apparent differential expression of proline-rich nuclear receptor coactivator 2 (PNRC2) by Illumina microarray is likely due to an artifact generated by polymorphisms in the region of the gene encoding the 3′-untranslated region (UTR) of the mRNA. A: gel of semiquantitative PCR to detect PNRC2 expression in WT and KO triceps surae, using primer sets designed to correspond to different regions of the 3′-UTR of the PNRC2 mRNA. B: representative of an alignment of sequenced amplicon from PNRC2 cDNA, from 1 of 3 WT animals vs. the GenBank-deposited Mus musculus PNRC2 gene. Samples from all 3 animals and 2 separate PCR reactions per animal yielded identical sequences to the example shown here. C: representative of an alignment of sequenced amplicon from PNRC2 cDNA, from 1 of 3 MuRF1 KO animals vs. the GenBank-deposited M. musculus PNRC2 gene. Samples from all 3 animals and 2 separate PCR reactions per animal yielded identical sequences to the example shown here. Boxed areas show 4 single base differences between the PNRC2 amplicon generated from MuRF1 KO animals and the GenBank sequence based on the C57/Bl6 genome. Arrows, up- and downstream PCR primer sites; over and underlined region, Illumina microarray PNRC2 probe site.