Fig. 5.

Effect of hM4Di activation plus forskolin application on larval heart rate. A: Background strain UAS-M4D and heart rate with solution changing; n = 6. B: Change of heart rate in UAS-M4D X 24B-GFP, n = 5, with the same solutions as in A. C: Comparison between background (dark grey) and M4D expressing group on the effect of CNO and forskolin (light grey). The differences between genotypes are represented by NS (not significant) or asterisk(s) above the lines. The differences, which are due to the changing one solution with another one in the same genotypes, are represented by NS or asterisk(s) above the bars. P ≤ 0.05 (*), P ≤ 0.02 (**), P ≤ 0.001 (***).