Fig. 9.

Quantitative comparison of areas activated by whisker stimulation in CxNR1KO and control animals and an exemplary dye spread in the cortex. A: activated area sizes are averaged after setting the threshold at half of the maximum signal strength. At its maximum, the activated area corresponded to 2,972 pixels in CxNR1KO cortex, significantly higher (t-test, 2-tailed, 2-sampled equal variants, P = 0.001301) than control cortices (1,137 pixels); the confidences are 591 and 202, respectively. B: example of RH-1691 dye spread in a coronal slice from the neocortex of a P22 mouse. The location of the fluorescence is over the D and E row of barrels, and the dye spans all cortical layers but is most prominent in layers II–IV. Cortical layers are indicated by Roman numerals. WM, white matter. Scale bar = 1 mm.