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. 2014 Jan 6;204(1):129–146. doi: 10.1083/jcb.201305084

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© 2014 Zambrano et al.

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Figure 3. — Transcriptionally active THRB mediates TH-induced senescence and DNA damage in MEFs. (A) Schematic representation of THRB, showing positions of the mutations C120G, AHT, and E452Q. DBD, DNA-binding domain; LBD, ligand-binding domain. (B) Thra/ThrbKO MEFs were transduced with vectors for the different mutants or H-Ras^V12, and after selection, THRB was detected by Western blotting. TUBA1A was used as a loading control. Black lines indicate the removal of an intervening lane for presentation purposes. (C) PDLs, estimated after one, two, and three passages (P < 0.0001, n = 3). (D) Percentages of SA-βgal⁺ cells after passage 3. (E) Percentage of cells having DNA damage foci determined from immunofluorescence of γ-H2AFX and TP53BP1 and merged images (P < 0.0001, n = 3). Results are presented as means ± SD. **, P < 0.01; ***, P < 0.001. WT, wild type.