Figure 4.

Glutamine-driven oxidative ATP production in normoxic and hypoxic cancer cells. (A) ATP production rates from oxidative phosphorylation and glycolysis in 4T1 and ASPC1 cancer cells. (B) Oxygen consumption rates in hypoxia measured after switching cells to complete media or media lacking glucose or glutamine for 8 h (mean±s.d. of N=3, significant differences (P<0.05) by T-test marked with *). (C) Contribution of oxidative phosphorylation to ATP production in NCI-60 cell lines as predicted by nutrient uptake rate-constrained flux balance analysis.