Figure 3.

MCP-1-deficiency in myeloid cells does not affect the concentration of MCP-1 or the accumulation of macrophages in peritoneal fluids in response to TG or zymosan A. (A,B) Peritoneal fluids were collected 4 h after i.p. injection of 1 ml of 3% TG (A) or 0.5 ml of 400 μg/ml zymosan A (B) and the concentration of MCP-1 in cell-free fluids was measured by ELISA. Data is presented as the mean ± SD obtained from the indicated number of mice. (C,D) Mice were i.p. injected with 1 ml of 3% TG (C) or 0.5 ml of 400 μg/ml zymosan A (D). Peritoneal cavities were flushed with 5 ml PBS 4 days after injection and the number of macrophages was counted. Data is presented as the mean ± SD. (E) Peritoneal resident cells were collected from WT C57BL/6 mice and transferred into the peritoneal cavity of MCP-1 KO mice. WT and MCP-1 KO mice adoptive transferred with WT peritoneal cells were i.p. injected with 1 ml of 3% TG. Peritoneal cavities were flushed with 5 ml PBS 4 h after injection and the concentration of MCP-1 in the peritoneal fluids was measured by ELISA. Data is presented as the mean ± SD obtained from three mice.