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MCP-1-deficiency in myeloid cells does not alter the production of MCP-1 in LPS-induced lung injury. Mice were exposed to LPS aerosol (100 or 1000 μg/ml in 10 ml PBS) for 30 min. Four or 24 h after LPS exposure, mice were euthanized by CO2 and BALFs were collected. (A) The expression of MCP-1 mRNA was examined by Northern blotting. (B) The number of macrophages was counted. Data is presented as the mean ± SD.
