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. 2014 Jan 7;4:213. doi: 10.3389/fneur.2013.00213

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Copyright © 2014 Cramer, Chatterjee, Lischka and Juliano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neurons can be successfully generated from passaged neurospheres even after multiple passages. (A) Representative example of neurons from passage 3 after 9 days in vitro immunopositive for the neuronal marker β-tubulin (green) and DAPI labeled cell nuclei (blue). The red arrow indicates a nucleus from a β-tubulin negative cell. (B) Group data for percentage of neurons identified as a function of neurosphere passage number. The percentage was highest in passage 1.