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Neurons generated by differentiating dissociated neurospheres express markers characteristic of different neocortical layers such as CUX1 (A), ER81 (B), and RORβ (C). In each example the layer-specific marker is indicated in red and the neuronal marker β-tubulin in green. (D–F) Group data depicting the distribution of marker expression across neurospheres passages one through three. Scale bar equals 50 μm.
