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SDS-PAGE analysis of HA-33 protein expression in E. coli BL21(DE3). Lanes 1-3, cell lysate of E. coli BL21(DE3) from three bacterial colonies containing pET28a-ha-33 gene after induction with IPTG; lane 4, cell lysate of E. coli BL21(DE3) containing pET28a-ha-33 gene before induction with IPTG; lane Mw, protein molecular weight markers. The expressed recombinant protein was observed at approximately 33 kDa
