Skip to main content
. Author manuscript; available in PMC: 2014 Jan 7.
Published in final edited form as: Proteomics. 2012 May;12(9):10.1002/pmic.201100633. doi: 10.1002/pmic.201100633

Figure 4. Western blot analysis confirms the proteomic results.

Figure 4

Depletion of SurA in JAS260 (surA depletion strain) leads to increased levels of BamA (compare lane 4 to lane 1), as reported in our previous proteomic analysis of that strain [21]. When SurA is depleted in the surA depletion skp strain (JAS261), BamA levels appear only slightly decreased (compare lane 6 to lane 3), in good agreement with our 2D-LC-MS/MS analysis where they are not changed. The levels of LamB and OmpA are greatly decreased when the surA depletion skp strain (JAS261) is grown under non-permissive conditions (compare lane 6 to lane 3), as observed in the 2D-LC-MS/MS experiments. The levels of all proteins examined remained largely unchanged in the skp strain (JAS417) (lanes 2 and 5).