LAT phosphorylation induces the binding of Grb2 to LAT Y171, Y191 and Y226. Grb2 simultaneously recruits several SH3 domain ligands to LAT. These include Sos1 and Sos2, which activated the MAP kinase pathway, c-Cbl, which facilitates the ubiquitination of multiple signalling proteins, and Gab2, which recruits and activates the phosphatase SHP-2 and inhibits the Gads/SLP-76 complex.