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Relative expression levels of OeLOX1 (A) and OeLOX2 (B) determined by qRT-PCR using the expression level of the genes in mature seed cotyledons as the calibrator. (C) Immunodetection of lipoxygenase (arrows). (D) In-gel assay of lipoxygenase activity (arrows). MS, mature dry seed; IM, seed after imbibition (24h); 6h and 3d, time of in vitro germination; 4 d, 8 d, 15 d, and 26 d, days of seedling growth.
