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. 2013 Oct 29;65(1):103–115. doi: 10.1093/jxb/ert355

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 8. — Detection of lipase (resorufin ester) and phospholipase A (BODIPY^® FL C₁₁-PC) activity and lipoxygenase (anti-LOX Ab) on OBs isolated from cotyledons dissected out from mature and imbibed seed. (A and B) Lipase activity visible as red fluorescence is located on the OB surface in imbibed seed (arrows). OBs isolated from mature seed do not show any labelling. (C and D) Green fluorescence is detected only on the surface of OBs (arrows) isolated from cotyledons after seed imbibition. (E and F) LOX is detected on the surface of OBs (arrows) in imbibed seed but it is absent in OBs isolated from cotyledons of mature seed. Bars=10 μm.