Fig. 1.

Responses of LeCDJ1 to chilling stress in tomato. (A) RNA gel blot analysis of the expression of LeCDJ1 in leaves treated at 4 °C in light (200 μmol m⁻² s⁻¹) for 0, 3, 6, 12, and 24h and then recovered at 25 °C for 2 and 4h. Ethidium bromide-stained rRNA is shown as a loading control. (B) qRT-PCR analysis of the response of LeCDJ1 to chilling stress in light. The transcript level of LeCDJ1 was normalized to EF-1α expression. Error bars represent the SD of triplicate reactions. (C) Western blot analysis of the LeCDJ1 protein levels in the leaves treated at 4 °C in light. LC, loading control (part of the Coomassie-stained total protein SDS-PAGE gel). (D) Quantitative image analysis of the protein content in (C) by Tanon Digital Gel Imaging Analysis System. The relative protein level of LeCDJ1 was normalized to the level at 0h.