Fig. 1.
Workflow model of the experimental conditions. Seeds were sowed on filter paper, and 3 days after germination seedlings were divided into four groups and transferred for 5 days to four different hydroponic solutions: +N solutions (+NO3 – and +NH4 + and –N solutions (NO3 – and NH4 +-depleted nutrient solution). After 5 days, seedlings were transferred to different nutrient solutions (+N solutions and –N solutions) and were treated for different times (30min, 2h, and 6h). At the end of the treatments, the eight groups were used to compare the effects of long- and short-term of nitrogen supply/depletion by means of a multifaceted transcriptomic approach.