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. 2013 Nov 12;65(1):185–200. doi: 10.1093/jxb/ert358

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© The Author 2013. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Design of the split-root system used to investigate the localized effects of nitrate on the intact root apex of maize seedlings. Seeds of maize inbred line B73 were sown on paper and then seedlings were transferred to a vertical plate system. Plate prepared with N-depleted solution and 1% agar were either supplied with 1mM nitrate (+N) or depleted (–N) by cutting and replacing a rounded portion of the agar, thus only apical portion of the root system could perceive the change of treatment. Seedlings continued to grow after the replacement of the rounded portion of agar, and at the end of the treatment they were removed from the system and harvested. M, meristem; TZ, transition zone; EZ, elongation zone; MZ, maturation zone.