. 2014 Jan 2;6:3. doi: 10.12703/P6-3

Table 1. Applications for RNA-guided Cas9 proteins.

Origin	Gene	Purpose	Application	Addgene #	Reference
Cas9^CSN, (H840A mutant) complementary strand nickase; Cas9^D, catalytically inactive/dead; Cas9^NSN, (D10A mutant) non-complementary strand nickase; CRISPRi, CRISPR-interference; DSB, double-strand DNA break; hCas9, mammalian codon optimized; hum/mus, human and mouse; SSN, single-strand nick; wCas9, worm codon optimized; zCas9, zebrafish codon optimized.
S. pyogenes	Cas9	DSB	in vitro	39312	[96]
	Cas9^CSN	SSN	in vitro	39316
	Cas9^NSN	SSN	in vitro	39315
	Cas9^D	dead	in vitro	39318
L. innocua	Cas9	DSB	in vitro	39313
S. thermophilus	Cas9	DSB	in vitro	39314
N. meningitidis	Cas9	DSB	in vitro	39317
S. pyogenes	hCas9	editing	hum/mus	42234	[22]
S. pyogenes	hCas9	editing	hum/mus	42230	[16]
	hCas9^NSN	editing	hum/mus	42335
	hCas9	editing	hum/mus	42229
	hCas9^NSN	editing	hum/mus	42333
S. pyogenes	hCas9	editing	hum/mus	41815	[25]
	hCas9^NSN	editing	hum/mus	41816
S. pyogenes	Cas9	editing	Bacteria	44250	[8]
	Cas9^D	CRISPRi	Bacteria	44249
	hCas9^D	CRISPRi	hum/mus	44246
	hCas9^D	CRISPRi	hum/mus	44247
S. pyogenes	Cas9	editing	Bacteria	42876	[7]
S. pyogenes	Cas9	editing	Zebrafish	42251	[35]
	Cas9	editing	hum/mus	42252
S. pyogenes	hCas9	editing	hum/mus	43945	[15]
S. pyogenes	hCas9	editing	hum/mus	44719	[18]
	hCas9^NSN	editing	hum/mus	44720
S. pyogenes	hCas9	editing	Yeast	43802	[11]
	hCas9	editing	Yeast	43804
S. pyogenes	hCas9	editing	hum/mus	44758	[59]
S. pyogenes	hCas9^D	tracking	hum/mus	46910	[10]
	hCas9^D	repression	hum/mus	46911
	hCas9^D	activation	hum/mus	46912
	hCas9^D	activation	hum/mus	46913
	hCas9^D	repression	Yeast	46920
	hCas9^D	repression	Yeast	46921
S. pyogenes	hCas9	editing	Drosophila	45945	[30]
	hCas9	editing	Drosophila	46294
S. pyogenes	wCas9	editing	C. elegans	46168	[41]
S. pyogenes	hCas9	editing	hum/mus	43861	Unpublished data (Joung Lab)
S. pyogenes	hCas9	editing	Plants	46965	[49]
S. pyogenes	zCas9	editing	Zebrafish	46757	[36]
	zCas9	editing	Zebrafish	47929
N. meningitidis	Cas9	editing	hum/mus	47867	[13]
S. pyogenes	wCas9	editing	C. elegans	47549	[46]
S. pyogenes	wCas9	editing	C. elegans	47911	[39]
S. pyogenes	wCas9	editing	C. elegans	47933	[42]